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Biochemical Engineering by James M. Lee

By James M. Lee

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1969. Chapra, S. C. and R. P. Canale, Numerical Methods for Engineers. , 1988. Charm, S. E. and B. L. Wong, “Enzyme Inactivation with Shearing,” Biotechnol. Bioeng. 12 (1970):1103–1109. Crueger, W. and A. Crueger, Biotechnology: A Textbook of Industrial Microbiology, pp. 161–162. , 1984. Eadie, G. , “The Inhibition of Cholinesterase by Physostigmine and Prostigmine,” J. Biol. Chem. 146 (1942):85–93. Eveleigh, D. , “The Microbiological Production of Industrial Chemicals,” Scientific American 245 (1981):155–178.

However, the further increases in the substrate concentration to 15mM decreased the initial reaction rate. This behavior may be due to substrate or product inhibition. Since the Michaelis-Menten equation does not incorporate the inhibition effects, we can drop the last two data points and limit the model developed for the low substrate concentration range only (CS ≤ 10mM). 8 shows the three plots prepared from the given data. The two data points which were not included for the linear regression were noted as closed circles.

38) = −KM + ln(CS0 / CS ) ln(CS0 / CS ) Enzyme Kinetics F CS0 2-25 .......... ... V, CS .......................................... 11 Schematic diagram of a continuous stirred-tank reactor (CSTR). The plot of (CS0-CS) /ln(CS0 /CS) versus t /ln(CS0 /CS) may yield a straight line with a slope of rmax and an intercept of –KM. 2. Continuous Stirred-Tank Reactor A continuous stirred-tank reactor (CSTR) is an ideal reactor which is based on the assumption that the reactor contents are well mixed. Therefore, the concentrations of the various components of the outlet stream are assumed to be the same as the concentrations of these components in the reactor.

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